Expansion of KO ES cells

What are Knockout-ES cells of the International Knockout Mouse Consortium?
Embryonic stem cells from the International Knockout Mouse Consortium ( www.knockoutmouse.org) are a fast and easy way to obtain "floxed" alleles. The laboratories of the consortium have planned to knockout most if not all mouse genes. Thousands of ES cell clones were already generated and the availability of a "floxed" allele can be checked in their database.

To generate knockout mice through IKMC, identify ES cell clones with targeted mutations in the gene of your interest. Order the ES cells (if possible 3 clones) from the respective service and have them send to the AGTF directly (make sure the bill goes to your address and not the AGTF). Do not have them send to your lab first!
It is advisable to join the Mouse Makers Club to design a strategy for confirming the integration into the appropriate locus once the cells have been expanded.

Short Description
This service offers the thawing and expansion of a ES cell clone. We will freeze at least 8 vials of the clone. Three vials and three cell pellets will be given to the researcher. The cell pellets can be used for preparation of DNA for Southern blot analysis or PCR. This service costs 1´200 CHF (+MWSt, non-USZ/UZH) per clone.

Prerequisites
- you have already identified "floxed" ES cells
- a completely filled out request form:

IKMC-ES-Cell-expansion-form.rtf (RTF, 3 MB)

Important
We normally try to process your request as soon as they arrive. In case of a backlog of requests, the date of DNA receipt and the number of requests submitted by individual groups will determine the sequence in which the projects are carried out.

In case of questions, please contact Thorsten Buch buch@immunology.uzh.ch (044 635 37 09) before submitting a request.